CP Baveja Microbiology PDF Free 248: The Ultimate Resource for Microbiology Students and Professiona

Flaming is done to inoculation loops and straight-wires in microbiology labs for streaking. Leaving the loop in the flame of a Bunsen burner or alcohol burner until it glows red ensures that any infectious agent is inactivated. This is commonly used for small metal or glass objects, but not for large objects (see Incineration below). However, during the initial heating, infectious material may be sprayed from the wire surface before it is killed, contaminating nearby surfaces and objects. Therefore, special heaters have been developed that surround the inoculating loop with a heated cage, ensuring that such sprayed material does not further contaminate the area. Another problem is that gas flames may leave carbon or other residues on the object if the object is not heated enough. A variation on flaming is to dip the object in a 70% or more concentrated solution of ethanol, then briefly touch the object to a Bunsen burner flame. The ethanol will ignite and burn off rapidly, leaving less residue than a gas flame

Figure 4. The inhibition approaches for LuxI/LuxR QS system. The signaling molecule AHL is produced by the luxI synthase gene and freely diffuses from each cell. When critical concentration is reached, the synthesized signal molecules diffuse back inside the bacterial cell and binds with LuxR. Then the QS transcription is activated by the LuxR-AHL complex. The target stages for inhibition are (1) Blockage of AHL molecule synthesis, (2) Degradation of the AHL molecule, and 3. Interference with the signal receptor.

cp baveja microbiology pdf free 248

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